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Real-time PCR To measure expression changes of apoptotic genes, total RNA from siRNA-targeted U2OS cells after 12 h continuous exposure to DNA damaging reagents at the same dose used in the Annexin V experiments was isolated using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol, and cDNA was generated from the isolated total RNA using ReverTra Ace qPCR RT master mix with gDNA remover (Toyobo, Osaka, Japan)
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Elevation of glutathione as a therapeutic strategy in Alzheimer disease
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