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Crypts ( N = 500) per well were seeded in 50 µl Matrigel (BD, 356231) on a pre-warmed 24-well plate and allowed to solidify for 10 min at 37 °C, after which 500 µl IntestiCult Growth Medium supplemented with 100U/ml penicillin and 100 µg/ml streptomycin (Biochrome, 0257F) was added

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, Xue W., Bar E., et al