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Gut and fat body samples were sequenced by the Max Planck Genome Center Cologne (MPGCC) on an Illumina HiSeq2500 Genome Analyzer platform, using paired-end (2 × 100 bp) reads

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Therefore, we next examined whether the expression levels of key genes involving environmental defenses could account for these observed phenotypes using both transgenic GFP animals as well as RT-qPCR