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For staining of intracellular cytokines, cells were stimulated in medium containing 1 μM ionomycin and 50 nM phorbol 12-myristate 13-acetate (PMA) in the presence of 5 μg/ml Brefeldin A at 37 °C for 4 h

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RiboCeine, his groundbreaking discovery, is the only scientifically proven compound that delivers intact cysteine into the cells