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A549 cells were cultured in DMEM/F12 supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 10 U/mL penicillin, and 10 µg/mL streptomycin
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The vial the clinic uses is 50 mg of GHK-Cu reconstituted with 2 mL of bacteriostatic water, which yields 25 mg/mL
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