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Related methods have assessed “total” H 2 S after its release by reduction of biological samples, followed by trapping with ethyliodoacetate 38 or monobromobimane 34,35
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We further investigated the protein levels of genes related to cysteine and GSH metabolism (CBS and CSE, two enzymes of the transsulfuration pathway, and GCLC and GCLM, both subunits of γ-GCS) in addition to GPX4 and FSP1, the two key ferroptosis regulatory nodes among different cell lines (Fig
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