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Protein concentrations in lysates were determined by Protein Assay Dye Reagent (BioRad #5000006), diluted in 2x Laemmli sample buffer (BioRad Cat#161–0737) with fresh β-mercaptoethanol (BioRad #1610710), and heated at 95°C for 5 minutes

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BPC-157 - Refined Bio (Type):Capsules

For each metabolic pathway, the median fold-change in the production of its metabolites was computed based on the pathway definition of ref