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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbecco’s Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37°C in a humidified environment with 5% CO 2
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These results collectively demonstrate that the advantages of supplements are not universal
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